Sample information curated by ChIP-Atlas

Antigen

Antigen Class
DNase-seq
Antigen
DNase-Seq

Cell type

Cell type Class
Unclassified
Cell type
Unclassified
NA
NA

Attributes by original data submitter

Sample

source_name
N2 L1 Arrest
strain
N2
Stage
L1 Arrest
library preparation
76 base pair single ended sequencing library
flowcell sample id
14138

Sequenced DNA Library

library_strategy
DNase-Hypersensitivity
library_source
GENOMIC
library_selection
DNase
library_construction_protocol
After treating with 45ug/mL boiled RNase A for 30 minutes, DNA was purified and concentrated using column purification. The DNA sample was run on 1% agarose, stained with SYBR Gold, and the gel piece containing DNA fragments less than 500bp was isolated and purified. DNA sample yield was measured using a Qubit fluorimeter. 50 base pair single ended sequencing library for embryo replicates B, C, D and L1 arrest X,Y,Z. 76 base pair single ended sequencing library for embryo replicate A and L1 arrest replicates W and V. Illumina HiSeq2500

Sequencing Platform

instrument_model
Illumina HiSeq 2500

ce11

Number of total reads
45399853
Reads aligned (%)
62.9
Duplicates removed (%)
46.5
Number of peaks
13642 (qval < 1E-05)

ce10

Number of total reads
45399853
Reads aligned (%)
62.9
Duplicates removed (%)
46.5
Number of peaks
13645 (qval < 1E-05)

Base call quality data from DBCLS SRA