Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MCRS1

Cell type

Cell type Class
Liver
Cell type
HuH-7
Primary Tissue
Liver
Tissue Diagnosis
Carcinoma Hepatocellular

Attributes by original data submitter

Sample

source_name
MCRS1_Huh7
cell line
Huh7
cell type
liver cancer cell line
chip antibody
MCRS1
chip antibody vendor
protein-tech
lot
1

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed in 0.3% formaldehyde for 30 minutes at 4 degrees prior to processing for ChIP using protocol from Raab et al 2015. Kapa Hyperprep kit using Illumina Truseq barcoded adapters

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
27512832
Reads aligned (%)
99.5
Duplicates removed (%)
0.4
Number of peaks
606 (qval < 1E-05)

hg38

Number of total reads
27512832
Reads aligned (%)
99.6
Duplicates removed (%)
0.4
Number of peaks
554 (qval < 1E-05)

Base call quality data from DBCLS SRA