Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Egr2

Cell type

Cell type Class
Blood
Cell type
CD4+ T cells
NA
NA

Attributes by original data submitter

Sample

source_name
CD4+ T cells_treatment with immobilized anti-CD3 mAb_Egr2_IP
strain
C57BL/6
cell type
CD4+ T cells
treatment
immobilized anti-CD3 mAb
chip antibody
EGR2 (PRB-236P,Covance)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and Egr2-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit

Sequencing Platform

instrument_model
Illumina Genome Analyzer

mm10

Number of total reads
21532321
Reads aligned (%)
57.8
Duplicates removed (%)
51.5
Number of peaks
13970 (qval < 1E-05)

mm9

Number of total reads
21532321
Reads aligned (%)
57.6
Duplicates removed (%)
51.8
Number of peaks
14028 (qval < 1E-05)

Base call quality data from DBCLS SRA