Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
CD4+ T cells
NA
NA

Attributes by original data submitter

Sample

source_name
CD4+ T cells_treatment with immobilized anti-CD3 mAb_input DNA
strain
C57BL/6
cell type
CD4+ T cells
treatment
immobilized anti-CD3 mAb
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and Egr2-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit

Sequencing Platform

instrument_model
Illumina Genome Analyzer

mm10

Number of total reads
4715970
Reads aligned (%)
73.3
Duplicates removed (%)
8.6
Number of peaks
47 (qval < 1E-05)

mm9

Number of total reads
4715970
Reads aligned (%)
73.0
Duplicates removed (%)
8.7
Number of peaks
57 (qval < 1E-05)

Base call quality data from DBCLS SRA