Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MORC2

Cell type

Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
V5 ChIP in MORC2 knockout cells
cell line
HeLa

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Formaldehye-crosslinked chromatin was sheared by sonication to an average size of ~300bp and then immunoprecipitated with the relevant antibodies: rabbit anti-TASOR (Atlas Antibodies, HPA006735), rabbit IgG (Cell Signaling, #2729) or anti-V5 agarose affinity gel (Sigma-Aldrich, A7345). NEBNext ChIP-seq Library Prep Kit (NEB)

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
62756566
Reads aligned (%)
65.4
Duplicates removed (%)
33.3
Number of peaks
1513 (qval < 1E-05)

hg38

Number of total reads
62756566
Reads aligned (%)
67.3
Duplicates removed (%)
31.9
Number of peaks
1658 (qval < 1E-05)

Base call quality data from DBCLS SRA