Formaldehye-crosslinked chromatin was sheared by sonication to an average size of ~300bp and then immunoprecipitated with the relevant antibodies: rabbit anti-TASOR (Atlas Antibodies, HPA006735), rabbit IgG (Cell Signaling, #2729) or anti-V5 agarose affinity gel (Sigma-Aldrich, A7345). NEBNext ChIP-seq Library Prep Kit (NEB)