Antigen

Antigen Class

TFs and others

Antigen

GFP

Cell type

Cell type Class

Neural

Cell type

Retina

MeSH Description

The ten-layered nervous tissue membrane of the eye. It is continuous with the OPTIC NERVE and receives images of external objects and transmits visual impulses to the brain. Its outer surface is in contact with the CHOROID and the inner surface with the VITREOUS BODY. The outer-most layer is pigmented, whereas the inner nine layers are transparent.

Sample

source_name

retina

tumor type

none

tissue

Epithelial cells immortalized with hTERT

chip antibody

Mouse anti-GFP, Cell Signaling #2955, lot 4B10

transgene

PGBD5-GFP

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-seq libraries were constructed with the NEBNext ChIP-seq library prep kit following the manufacturer’s recommended protocol (New England Biolabs, Ipswich, MA, USA).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

54675428

Reads aligned (%)

84.7

Duplicates removed (%)

8.2

Number of peaks

587 (qval < 1E-05)

hg19

Number of total reads

54675428

Reads aligned (%)

84.0

Duplicates removed (%)

8.4

Number of peaks

371 (qval < 1E-05)