Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Others
Cell type
Pleomorphic sarcoma
NA
NA

Attributes by original data submitter

Sample

source_name
UPS_input
cell type
Undifferentiated Pleomorphic Sarcoma (UPS)
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Samples were homogenized with rotor-stator device. Lysates were sonicated via Bioruptor. Protein-DNA complexes were isolated via immunoprecipitation and prepared for sequencing with Rubicon Thruplex DNA-seq kit. ChIP-seq via single-end 75-bp sequencing on Nextseq 500.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
53612168
Reads aligned (%)
97.4
Duplicates removed (%)
4.9
Number of peaks
1196 (qval < 1E-05)

hg19

Number of total reads
53612168
Reads aligned (%)
96.7
Duplicates removed (%)
6.2
Number of peaks
1211 (qval < 1E-05)

Base call quality data from DBCLS SRA