Antigen

Antigen Class

TFs and others

Antigen

HMBOX1

Cell type

Cell type Class

Uterus

Cell type

HeLa

Primary Tissue

Cervix

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

HeLa 1.2.11 ALT (-) cells, HMBOX1 ChIP

cell line

HeLa

alt status

-

chip antibody

anti-HMBOX1 / Rabbit polyclonal IgG (Abcam ab97643)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared by Fasteris SA (Switzerland) and were sequenced on HiSeq 2000.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

25785720

Reads aligned (%)

98.4

Duplicates removed (%)

5.0

Number of peaks

1265 (qval < 1E-05)

hg19

Number of total reads

25785720

Reads aligned (%)

97.6

Duplicates removed (%)

6.5

Number of peaks

1328 (qval < 1E-05)