Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
HMBOX1

Cell type

Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
HeLa 1.2.11 ALT (-) cells, HMBOX1 ChIP
cell line
HeLa
alt status
-
chip antibody
anti-HMBOX1 / Rabbit polyclonal IgG (Abcam ab97643)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared by Fasteris SA (Switzerland) and were sequenced on HiSeq 2000.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
25785720
Reads aligned (%)
91.2
Duplicates removed (%)
6.5
Number of peaks
1328 (qval < 1E-05)

hg38

Number of total reads
25785720
Reads aligned (%)
93.4
Duplicates removed (%)
5.0
Number of peaks
1265 (qval < 1E-05)

Base call quality data from DBCLS SRA