Antigen

Antigen Class

Histone

Antigen

H2A.Z

Cell type

Cell type Class

Blood

Cell type

Monocytes-CD14+

Tissue

monocytes

Lineage

mesoderm

Description

Monocytes-CD14+ are CD14-positive cells from human leukapheresis production, from donor RO 01746 (draw 1 ID is RO 01746, draw 2 ID is RO 01826), newly promoted to tier 2: not in 2011 analysis

Sample

source_name

T11, 1st pair, 1st individual

cell type

CD14 cells

individual identifier

T11

chip antibody

anti-H2A.Z (Active Motif, Cat no: 39113)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

The chromatin immunoprecipitation (ChIP) assay was performed on 5 x 105 CD14+ cells according to previously published protocols. Chromatin was sonicated to get fragments of 100-500 bp and immunoprecipitated with 10 ul of anti-H2A.Z antibody (Active Motif, Cat no: 39113). ChIP-seq libraries were prepared following the Illumina protocol and ligated to standard PE adaptors and sequenced on an Illumina GAIIx instrument.

Sequencing Platform

instrument_model

Illumina Genome Analyzer IIx

hg38

Number of total reads

43797728

Reads aligned (%)

88.6

Duplicates removed (%)

19.3

Number of peaks

30400 (qval < 1E-05)

hg19

Number of total reads

43797728

Reads aligned (%)

88.0

Duplicates removed (%)

19.6

Number of peaks

29840 (qval < 1E-05)