Curated Sample Data


Genome
dm3
Antigen Class
Input control
Antigen
Input control
Cell type Class
Adult
Cell type
Testis

Cell type information


NA
NA

Attributes by Original Data Submitter


source_name
Input_for_H3K27_in_comr
genotype
comr
gender
male
age
3 days old
tissue
adult testes
chip antibody
none

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
To perform H3K27me3 ChIP-seq, we used the True MicroChIP & MicroPlex Library Preparation™ Package (Diagenode), according to manufacturer’s recommendations. 400 adult testes of comr or can homozygotes were used as a starting material for two biological replicates. Abcam anti-H3K27me3 antibodies (#6002) were used for chromatin immunoprecipitation. Chromatin was sheared using BioRuptor instrument (Diagenode). DNA from precipitated material and input were used for library preparation using MicroPlex Library Preparation protocol. The libraries were sequenced on the Illumina MiSeq system.

Platform Information


instrument_model
Illumina MiSeq

External Database Query

Logs in read processing pipeline


Number of total reads
1686477
Reads aligned (%)
93.7
Duplicates removed (%)
7.9
Number of peaks
691 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA