Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Adult
Cell type
Testis
NA
NA

Attributes by original data submitter

Sample

source_name
Input_for_H3K27_in_can
genotype
can
gender
male
age
3 days old
tissue
adult testes
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
To perform H3K27me3 ChIP-seq, we used the True MicroChIP & MicroPlex Library Preparation™ Package (Diagenode), according to manufacturer’s recommendations. 400 adult testes of comr or can homozygotes were used as a starting material for two biological replicates. Abcam anti-H3K27me3 antibodies (#6002) were used for chromatin immunoprecipitation. Chromatin was sheared using BioRuptor instrument (Diagenode). DNA from precipitated material and input were used for library preparation using MicroPlex Library Preparation protocol. The libraries were sequenced on the Illumina MiSeq system.

Sequencing Platform

instrument_model
Illumina MiSeq

dm6

Number of total reads
3077634
Reads aligned (%)
85.5
Duplicates removed (%)
22.0
Number of peaks
499 (qval < 1E-05)

dm3

Number of total reads
3077634
Reads aligned (%)
86.8
Duplicates removed (%)
17.3
Number of peaks
977 (qval < 1E-05)

Base call quality data from DBCLS SRA