Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
Embryonic stem cells
cell type
Embryonic stem cells
strain
C57BL/6-129/Sv
passages
10-20
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Soluble chromatin lysates were extracted from sonicated nuclei and protein-DNA complexes were immunoprecipitated with antibody. The libraries were prepared from 10 ng of immunoprecipiated DNA or input DNA using Illumina ChIP-seq Sample Preparation Kit and purified with a Qiagen DNA Isolation column.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
59639797
Reads aligned (%)
83.9
Duplicates removed (%)
9.4
Number of peaks
2183 (qval < 1E-05)

mm9

Number of total reads
59639797
Reads aligned (%)
83.8
Duplicates removed (%)
9.4
Number of peaks
2162 (qval < 1E-05)

Base call quality data from DBCLS SRA