Antigen

Antigen Class

TFs and others

Antigen

SMARCE1

Cell type

Cell type Class

Breast

Cell type

HMLE

NA

NA

Sample

source_name

HMLE-Twist-ER induced to undergo an EMT

chip antibody

SMARCE1 (Abcam, ab70540)

cell type

HMLE-Twist-ER_125nM

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Nuclei were sonicated to retrieve lysates and protein-DNA complexes were isolated with antibody. To make libraries, TruSeq ChIP protocol was followed.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

56651712

Reads aligned (%)

96.2

Duplicates removed (%)

94.5

Number of peaks

228 (qval < 1E-05)

hg19

Number of total reads

56651712

Reads aligned (%)

95.4

Duplicates removed (%)

95.9

Number of peaks

228 (qval < 1E-05)