Antigen

Antigen Class

TFs and others

Antigen

SMARCC1

Cell type

Cell type Class

Breast

Cell type

HMLE

NA

NA

Sample

source_name

HMLE-Twist-ER Ctrl

chip antibody

SMARCC1 (Santa Cruz, sc-9746)

cell type

HMLE-Twist-ER_0nM

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Nuclei were sonicated to retrieve lysates and protein-DNA complexes were isolated with antibody. To make libraries, TruSeq ChIP protocol was followed.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg19

Number of total reads

23410583

Reads aligned (%)

54.8

Duplicates removed (%)

32.1

Number of peaks

633 (qval < 1E-05)

hg38

Number of total reads

23410583

Reads aligned (%)

56.2

Duplicates removed (%)

31.1

Number of peaks

477 (qval < 1E-05)