Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SMARCC1

Cell type

Cell type Class
Breast
Cell type
HMLE
NA
NA

Attributes by original data submitter

Sample

source_name
HMLE-Twist-ER Ctrl
chip antibody
SMARCC1 (Santa Cruz, sc-9746)
cell type
HMLE-Twist-ER_0nM

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Nuclei were sonicated to retrieve lysates and protein-DNA complexes were isolated with antibody. To make libraries, TruSeq ChIP protocol was followed.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
23410583
Reads aligned (%)
54.8
Duplicates removed (%)
32.1
Number of peaks
633 (qval < 1E-05)

hg38

Number of total reads
23410583
Reads aligned (%)
56.2
Duplicates removed (%)
31.1
Number of peaks
477 (qval < 1E-05)

Base call quality data from DBCLS SRA