Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
STAT3

Cell type

Cell type Class
Blood
Cell type
Th17 Cells
MeSH Description
A subset of helper-effector T-lymphocytes which synthesize and secrete INTERLEUKINS IL-17; IL-17F; and IL-22. These cytokines are involved in host defenses and tissue inflammation in autoimmune diseases.

Attributes by original data submitter

Sample

source_name
Th17_STAT3
tissue
healthy neonates
tissue
Umbilical cord blood
cell type
primary human CD4+ T cells
chip antibody
polyclonal anti-STAT3 antibody (rabbit polyclonal αSTAT3 9132L, Cell Signaling Technology)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed using formaldehyde solution, lysed and sonicated to obtain chromatin fragments of 100-500bp size. The sonicated chromatin was then subjected to immunoprecipitation with polyclonal anti-STAT3 antibody (rabbit polyclonal αSTAT3 9132L, Cell Signaling Technology). The STAT3 bound chromatin was isolated using Dynal magnetic beads coated with secondary antibody anti-rabbit IgG (Dynal Biotech). The immunoprecipitated DNA was eluted, reverse crosslinked and purified using Qiagen minielute columns. The sample preparation for sequencing was performed according to Illumina's recommendations by Illumina service provider, Fasteris Life Sciences, Switzerland. Having passed quality control, the library is sequenced on Illumina HiSeq 2500

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
22689414
Reads aligned (%)
74.4
Duplicates removed (%)
15.2
Number of peaks
823 (qval < 1E-05)

hg38

Number of total reads
22689414
Reads aligned (%)
75.9
Duplicates removed (%)
14.5
Number of peaks
686 (qval < 1E-05)

Base call quality data from DBCLS SRA