Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SPI1

Cell type

Cell type Class
Blood
Cell type
ME-1
Primary Tissue
Bone Marrow
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
ME-1 cell line (leukemic)
cell type
leukemic cell line
cell line
ME-1
antibody target
PU.1
antibody company
Santa Cruz
antibody catalogue number
sc-22805
treatment
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was sonicated and used for ChIP seq using specific antibodies (avialable on suplementry table). Libraries were prepared according to Illumina's standard protocol.

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

hg19

Number of total reads
25193225
Reads aligned (%)
16.7
Duplicates removed (%)
81.9
Number of peaks
6193 (qval < 1E-05)

hg38

Number of total reads
25193225
Reads aligned (%)
17.2
Duplicates removed (%)
81.5
Number of peaks
6168 (qval < 1E-05)

Base call quality data from DBCLS SRA