GSM2538840: Input DNA [H21792]; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Pluripotent stem cell
Cell type
iPS cells
NA
NA
Attributes by original data submitter
Sample
source_name
induced pluripotent stem cells, input
cell line
H21792
cell type
induced pluripotent stem cells
Sex
female
population
Caucasian
chip antibody
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq experiments were largely performed according to a published protocol (Schmidt et al. 2009). Briefly, 30 million cells were cross-linked with 1% formaldehyde for 10 min at room temperature followed by quenching with 2.5 M glycine. Following cell lysis and sonication (Covaris S2: 4 min, duty cycle 10%, 5 intensity, 200 cycles per burst in 4x 6 x 16 mm tubes per individual), lysates were incubated with 5 μg H3K9me3 antibody (8898, Abcam) overnight. Libraries were prepared using the Illumina TruSeq ChIP Sample Preparation kit. Libraries were sequenced on the HiSeq2500 following the manufacturer's protocols.