Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Cardiovascular

Cell type

Heart Ventricles

MeSH Description

The lower right and left chambers of the heart. The right ventricle pumps venous BLOOD into the LUNGS and the left ventricle pumps oxygenated blood into the systemic arterial circulation.

Sample

source_name

Control

strain

C57BL/6

genotype/variation

control

tissue

Left ventricle

cell type

Isolated cardiac myocytes

chip antibody

none (input)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP for CTCF (Active Motif Cat# 61311) was performed on pooled isolated cardiomyocytes (~10 million total), using the ChIP-IT High Sensitivity Kit as described (Monte et al., 2016). Cardiomyocyte pools were obtained from 3 control and 3 CTCF KO hearts. ChIP-seq libraries were prepared using the NuGEN Ovation Ultralow System V2 1–16 kit (Cat# 0344).

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

66486250

Reads aligned (%)

97.3

Duplicates removed (%)

18.5

Number of peaks

700 (qval < 1E-05)

mm9

Number of total reads

66486250

Reads aligned (%)

97.0

Duplicates removed (%)

18.4

Number of peaks

790 (qval < 1E-05)