Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
Embryonic stem cells
cell type
Embryonic stem cells
passages
10-20
strain
129/Ola
antibody
H3 (Abcam, ab1791)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP libraries were prepared using the NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs) according to manufacturer's instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 1000

mm10

Number of total reads
13362539
Reads aligned (%)
97.3
Duplicates removed (%)
11.3
Number of peaks
363 (qval < 1E-05)

mm9

Number of total reads
13362539
Reads aligned (%)
97.1
Duplicates removed (%)
11.4
Number of peaks
360 (qval < 1E-05)

Base call quality data from DBCLS SRA