Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
Embryonic stem cells
cell type
Embryonic stem cells
passages
10-20
strain
129/Ola
antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP libraries were prepared using the NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs) according to manufacturer's instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 1000

mm10

Number of total reads
18916863
Reads aligned (%)
96.5
Duplicates removed (%)
10.4
Number of peaks
350 (qval < 1E-05)

mm9

Number of total reads
18916863
Reads aligned (%)
96.3
Duplicates removed (%)
10.4
Number of peaks
353 (qval < 1E-05)

Base call quality data from DBCLS SRA