Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Adult
Cell type
Muscle
NA
NA

Attributes by original data submitter

Sample

source_name
fly muscle
tissue
muscle
age
30 day
genotype
5905
chip antibody
H3K27me3 (Millipore 07-449)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-Seq, 5% mouse epigenome was used as internal control for H3K27me3 IP. For RNA-Seq, RNA was isolated by TRIZol reagent, and polyA tail RNAs were selected. For small RNA-seq, ~20-29 nt small RNA was recoveried by 15% TBE-UREA PAGE gels. ChIP libraries were prepared according to instructions of NEB DNA library prep kit (NEB, E7370L). RNA libraries were prepared according to instructions of NEB Ultra RNA library prep kit (NEB, E7530L). Small RNA libraries were prepared by NEBNext Multiplex Small RNA Library Prep Set for Illumina (NEB, E7300S). ChIP-Seq or RNA-Seq or miRNA-seq

Sequencing Platform

instrument_model
Illumina MiSeq

dm6

Number of total reads
2360750
Reads aligned (%)
92.6
Duplicates removed (%)
1.8
Number of peaks
427 (qval < 1E-05)

dm3

Number of total reads
2360750
Reads aligned (%)
92.9
Duplicates removed (%)
1.1
Number of peaks
180 (qval < 1E-05)

Base call quality data from DBCLS SRA