Antigen

Antigen Class

TFs and others

Antigen

HOXB13

Cell type

Cell type Class

Prostate

Cell type

22Rv1

Primary Tissue

Prostate

Tissue Diagnosis

Carcinoma

Sample

source_name

22Rv1 prostate cancer cells, HOXB13 ChIP

cell line

22Rv1

cell type

prostate cancer cell line

passage

< 25

chip antibody

HOXB13 (Santa Cruz Biotechnology cat. sc-66923)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Genomic DNA was extracted following sonication, clearing of lysates, and immunoprecipitation of protein/DNA complexes. Libraries were constructed using Rubicon Thruplex FD kit using manufacturer's recommended protocol.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg19

Number of total reads

18281023

Reads aligned (%)

97.9

Duplicates removed (%)

7.5

Number of peaks

2280 (qval < 1E-05)

hg38

Number of total reads

18281023

Reads aligned (%)

98.6

Duplicates removed (%)

6.2

Number of peaks

2202 (qval < 1E-05)