Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
HOXB13

Cell type

Cell type Class
Prostate
Cell type
22Rv1
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
22Rv1 prostate cancer cells, HOXB13 ChIP
cell line
22Rv1
cell type
prostate cancer cell line
passage
< 25
chip antibody
HOXB13 (Santa Cruz Biotechnology cat. sc-66923)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Genomic DNA was extracted following sonication, clearing of lysates, and immunoprecipitation of protein/DNA complexes. Libraries were constructed using Rubicon Thruplex FD kit using manufacturer's recommended protocol.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
15402339
Reads aligned (%)
89.3
Duplicates removed (%)
9.1
Number of peaks
1591 (qval < 1E-05)

hg38

Number of total reads
15402339
Reads aligned (%)
91.3
Duplicates removed (%)
7.7
Number of peaks
1562 (qval < 1E-05)

Base call quality data from DBCLS SRA