Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
tatsf1floxCreERT2 embryonic stem cells
treatment
Tamoxifen
barcode
2:CGATGT
treatment time
48h
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin IP was performed using anti-Polymerase II (Pol II N-20 (Santa Cruz sc-899) ) antibody. Precipitated genomic DNA or whole genomic DNA was used for library preparation. Library preparation was done using Kapa Library Preparation Kit (Roche). Barcoded samples were multiplexed and sequenced 50bp SE on a HighSeq2500 (Illumina).

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
56293633
Reads aligned (%)
98.6
Duplicates removed (%)
12.2
Number of peaks
606 (qval < 1E-05)

mm9

Number of total reads
56293633
Reads aligned (%)
98.3
Duplicates removed (%)
12.2
Number of peaks
682 (qval < 1E-05)

Base call quality data from DBCLS SRA