GSM2536358: tat tam input 1; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
tatsf1floxCreERT2 embryonic stem cells
treatment
Tamoxifen
barcode
2:CGATGT
treatment time
48h
chip antibody
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin IP was performed using anti-Polymerase II (Pol II N-20 (Santa Cruz sc-899) ) antibody. Precipitated genomic DNA or whole genomic DNA was used for library preparation. Library preparation was done using Kapa Library Preparation Kit (Roche). Barcoded samples were multiplexed and sequenced 50bp SE on a HighSeq2500 (Illumina).