Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
tatsf1floxCreERT2 embryonic stem cells
treatment
no treatment
barcode
6:GCCAAT
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin-IP was performed with rabbit anti mouse HTATSF1 antibody. Library preparation was done using Kapa Library Preparation Kit (Roche). Barcoded samples were multiplexed and sequenced 50bp SE on a HighSeq2500 (Illumina).

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
35367367
Reads aligned (%)
98.0
Duplicates removed (%)
17.4
Number of peaks
559 (qval < 1E-05)

mm9

Number of total reads
35367367
Reads aligned (%)
97.8
Duplicates removed (%)
17.4
Number of peaks
595 (qval < 1E-05)

Base call quality data from DBCLS SRA