Antigen

Antigen Class

Histone

Antigen

H3.3

Cell type

Cell type Class

Blood

Cell type

DOHH-2

Primary Tissue

Blood

Site of Extraction

Effusion, Pleural

Tissue Diagnosis

Lymphoma B-cell

Sample

dev stage

adult

cell line

DOHH2

sex

male

sample type

immortalized cell line

donor ID

ENCDO352AAA

age

60 year

health state

refractory immunoblastic B cell lymphoma progressed from follicular centroblastic/centrocytic lymphoma

lab

Bradley Bernstein, Broad

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

general protocol: https://www.encodeproject.org/documents/be2a0f12-af38-430c-8f2d-57953baab5f5/@@download/attachment/Epigenomics_Alternative_Mag_Bead_ChIP_Protocol_v1.1_exp.pdf

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

44996207

Reads aligned (%)

96.3

Duplicates removed (%)

10.4

Number of peaks

1270 (qval < 1E-05)

hg19

Number of total reads

44996207

Reads aligned (%)

95.4

Duplicates removed (%)

10.6

Number of peaks

647 (qval < 1E-05)