Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Embryo
Cell type
Embryonic brains
NA
NA

Attributes by original data submitter

Sample

source_name
cortex
developmental stage
embryonic day 14-15
chip antibody
none
tissue
cortex
strain
CD1

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysate from sonicated cortices were cleared after centrifugation. Foxg1-DNA complexes were isolated using a Foxg1 antibody. Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Part# 0801-0303). ChIP-Seq library was sequenced for pair-end sequencing (51 nt per end) on an Illumina Genome Analyzer II platform

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

mm10

Number of total reads
11449807
Reads aligned (%)
97.6
Duplicates removed (%)
2.6
Number of peaks
266 (qval < 1E-05)

mm9

Number of total reads
11449807
Reads aligned (%)
97.5
Duplicates removed (%)
3.0
Number of peaks
223 (qval < 1E-05)

Base call quality data from DBCLS SRA