Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Bone

Cell type

EWS502

NA

NA

Sample

source_name

Ewing sarcoma cell line

cell line

EWS502

tissue

Gastrointestinal stromal tumor (GIST) cell line

chip antibody

H3K27ac, Abcam ab4729

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. Libraries for single-end 75 bp sequencing on an Illumina NextSeq 500 were prepared with a ThruPLEX DNA-seq Kit (Rubicon) for ChIP-seq or Nextera DNA Preparation Kit (Illumina) for ATAC-seq.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

66207495

Reads aligned (%)

98.3

Duplicates removed (%)

4.8

Number of peaks

16265 (qval < 1E-05)

hg19

Number of total reads

66207495

Reads aligned (%)

97.9

Duplicates removed (%)

5.5

Number of peaks

16060 (qval < 1E-05)