Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Blood

Cell type

Lymphoblastoid cell line

Tissue

blood

Lineage

mesoderm

Description

parental cell type to lymphoblastoid cell lines

Sample

source_name

B-LCL_Ctrl2

cell type

cell line

genotype

DNMT3BA603T/wt

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei. Histone-DNA and DNMT3B-DNA complexes were isolated with specific antibodies. Immunoprecipitated complexes were recovered with protein A sepharose (Pharmacia), washed with low and high salt buffers, reverse-crosslinked, and purified. DNMT3B and H3K36me3 libraries were prepared according to Illumina's instructions accompanying the Nextera DNA Sample Kit. H3K27me3 and H3K4me3 libraries were prepared according to SOLiD protocol (Applied Biosystem).

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

34323667

Reads aligned (%)

98.7

Duplicates removed (%)

2.5

Number of peaks

868 (qval < 1E-05)

hg19

Number of total reads

34323667

Reads aligned (%)

97.7

Duplicates removed (%)

3.7

Number of peaks

1209 (qval < 1E-05)