Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase II

Cell type

Cell type Class

Blood

Cell type

MOLT-4

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Lymphocytic

Sample

source_name

T-ALL

cell line

MOLT4

antibody

RNA Pol II

antibody manufacturer

Diagenode

antibody catalog number

C15100055

drug treatment

dBET6 (100 nM) for 2 hours

input sample

MOLT4_Pol2_input

sequencing barcode

TTCCTGCT

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Standard DNA extraction from MOLT4 cell lines using cell lysis. Probe sonicator 3 minutes total sonication time (1 sec on, 4 sec off, 60% amplitude) on ice. Library prep with Rubicon TruePlex kit.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

31673415

Reads aligned (%)

71.4

Duplicates removed (%)

26.1

Number of peaks

16698 (qval < 1E-05)

hg19

Number of total reads

31673415

Reads aligned (%)

70.0

Duplicates removed (%)

25.2

Number of peaks

16567 (qval < 1E-05)