Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Blood
Cell type
Naive T cells
NA
NA

Attributes by original data submitter

Sample

source_name
naive CD8 T cells
strain
C57BL/6
age
six to seven weeks
tissue
Spleen

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Splenocytes were isolated and specific CD8 T cell subsets were sorted by FACS ATAC-seq library was constructed by Nextera DNA Sample Prep Kit (Illumina). For ChIP-seq library, DNA was end-repaired using End-it End-repair kit (Epicentre) and then added an “A” base to the 3’ end of DNA fragments using Klenow (NEB). Then DNA was ligated with adaptors using quick DNA ligase (NEB) at 25°C for 15 min followed by size selection of 200-400 bp using AMPure SPRI beads (Beckman Coulter). The adaptor ligated DNA was amplified using NEBNext High-Fidelity 2X PCR master mix (NEB).

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
28430510
Reads aligned (%)
98.7
Duplicates removed (%)
10.6
Number of peaks
18811 (qval < 1E-05)

mm9

Number of total reads
28430510
Reads aligned (%)
98.5
Duplicates removed (%)
10.7
Number of peaks
18770 (qval < 1E-05)

Base call quality data from DBCLS SRA