Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Neural
Cell type
Frontal cortex
NA
NA

Attributes by original data submitter

Sample

source_name
Frontal cortex
cell type
Camk2a+ excitatory cells
strain
129/FVB
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Nuclear lysates were sonicated and Flag-H1.0-DNA complexes were isolated with Anti-Flag antibody. Library was prepared by Genomic and RNA Profiling Core at Baylor College of Medicine using ThruPLEX DNA-seq kit (Rubicon).

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
115849765
Reads aligned (%)
93.0
Duplicates removed (%)
11.1
Number of peaks
1465 (qval < 1E-05)

mm9

Number of total reads
115849765
Reads aligned (%)
92.9
Duplicates removed (%)
11.2
Number of peaks
1594 (qval < 1E-05)

Base call quality data from DBCLS SRA