Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MPHOSPH8

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
ChIP:MPP8_Cell:TASOR-KO
cell line
K562
cell type
Chronic myelogenous leukemia
genotype/variation
TASOR KO
chip antibody
MPP8 (16796-1-AP, Protein Technologies Inc)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Total RNA was isolated from K562 cells using the RNAeasy kit (Qiagen # 74104) and treated with RNase-Free DNase Set (Qiagen # 79254) to remove genomic DNA, according to the manufacturer’s instructions. ChIP DNA, input DNA or cDNA were subject to end repair, A-tailing, adaptor ligation and cleavage with USER enzyme, followed by size selection to 250-500 bp and amplification with NEBNext sequencing primers. Libraries were purified, quantified, multiplexed and sequenced with 1x75bp single-end reads on an Illumina NEXT-seq (Stanford Functional Genomics Facility). library strategy: CRISPR screen, ChIP-seq and RNA-seq

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
26880948
Reads aligned (%)
182.4
Duplicates removed (%)
6.3
Number of peaks
454 (qval < 1E-05)

hg38

Number of total reads
26880948
Reads aligned (%)
184.2
Duplicates removed (%)
6.2
Number of peaks
718 (qval < 1E-05)

Base call quality data from DBCLS SRA