GSM2508064: 4-KMT2D ChIP-seq BYL719; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
KMT2D
Cell type
Cell type Class
Breast
Cell type
T-47D
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal
Attributes by original data submitter
Sample
source_name
Breast Cancer Cell
cell line
T47D cell
passages
5 to 10
chip antibody
Anti-KMT2D, HPA035977, Sigma
treatment
BYL719 1uM for 4hr
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were lysed and sonicated leading to a DNA average size of 200bp. Protein G dynabeads were pre-incubated with 5μg of KMT2D antibody (HPA035977, Sigma) with pre cleared chromatin. The complexes were reverse cross-linked and DNA was purified. Due to low enrichment due to antibody lot issues, ChIP-seq libraries were prepared using the total 5 ng of DNA and Illumina's TruSeq ChIP sample prep.Libraries were validated using the Agient Technologies 2100 Bioanalyzer and Qubit high sensitivity assay.