Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Gonad
Cell type
Spermatogenic cells
NA
NA

Attributes by original data submitter

Sample

source_name
spermatogenic cells, Ezh2 mutant, input
strain/background
mixed background
genotype/variation
MvhCre;Ezh2fl/fl
age
6 weeks
cell type
spermatogenic cells
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Testes were collected from 6-week-old mice and digested with collagenase, trypsin, and DNaseI. Percoll solution and cell strainers were applied to separate somatic cells from spermatogenic cells. The isolated cells were examined under differential interference contrast optics and 90% of them were spermatogenic cells. ChIP-seq libraries were prepared according to Illumina instructions and sequenced on Illumina's HiSeq2000 instrument.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
59078150
Reads aligned (%)
97.9
Duplicates removed (%)
19.1
Number of peaks
586 (qval < 1E-05)

mm9

Number of total reads
59078150
Reads aligned (%)
97.6
Duplicates removed (%)
19.0
Number of peaks
641 (qval < 1E-05)

Base call quality data from DBCLS SRA