Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Bone
Cell type
U2OS
Tissue
bone
Lineage
mesoderm
Description
osteosarcoma from the tibia of a 15 year old, J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma, viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses, mycoplasma contamination was detected and eliminated in 1972, (PMID: 6081590)

Attributes by original data submitter

Sample

collection_date
2010-11-17
cell_line
U2OS_GRalpha
cell_type
Human osteosarcoma cell line
sample_type
cell culture
treatment
90 min dexamethasone
antibody
Input
target_protein
NA

Sequenced DNA Library

library_name
GRalpha_input_90m_dex_BS
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
79857128
Reads aligned (%)
91.3
Duplicates removed (%)
9.8
Number of peaks
2448 (qval < 1E-05)

hg19

Number of total reads
79857128
Reads aligned (%)
90.2
Duplicates removed (%)
10.4
Number of peaks
818 (qval < 1E-05)

Base call quality data from DBCLS SRA