Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NR3C1

Cell type

Cell type Class
Bone
Cell type
U2OS
Tissue
bone
Lineage
mesoderm
Description
osteosarcoma from the tibia of a 15 year old, J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma, viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses, mycoplasma contamination was detected and eliminated in 1972, (PMID: 6081590)

Attributes by original data submitter

Sample

collection_date
17-Nov-2010
cell_line
U2OS_A477T
cell_type
Human osteosarcoma cell line
sample_type
cell culture
treatment
90 min dexamethasone
antibody
N499
target_protein
Glucocorticoid receptor

Sequenced DNA Library

library_name
A477T_ChIP_90m_dex
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

hg19

Number of total reads
26902849
Reads aligned (%)
79.7
Duplicates removed (%)
41.1
Number of peaks
36650 (qval < 1E-05)

hg38

Number of total reads
26902849
Reads aligned (%)
80.9
Duplicates removed (%)
40.6
Number of peaks
36996 (qval < 1E-05)

Base call quality data from DBCLS SRA