Antigen

Antigen Class

TFs and others

Antigen

NR3C1

Cell type

Cell type Class

Bone

Cell type

U2OS

Tissue

bone

Lineage

mesoderm

Description

osteosarcoma from the tibia of a 15 year old, J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma, viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses, mycoplasma contamination was detected and eliminated in 1972, (PMID: 6081590)

Sample

collection_date

2010-11-17

cell_line

U2OS_A477T

cell_type

Human osteosarcoma cell line

sample_type

cell culture

treatment

90 min dexamethasone

antibody

N499

target_protein

Glucocorticoid receptor

Sequenced DNA Library

library_name

A477T_ChIP_90m_dex

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer II

hg19

Number of total reads

26902849

Reads aligned (%)

79.7

Duplicates removed (%)

41.1

Number of peaks

36650 (qval < 1E-05)

hg38

Number of total reads

26902849

Reads aligned (%)

80.9

Duplicates removed (%)

40.6

Number of peaks

36996 (qval < 1E-05)