Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Bone

Cell type

U2OS

Tissue

bone

Lineage

mesoderm

Description

osteosarcoma from the tibia of a 15 year old, J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma, viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses, mycoplasma contamination was detected and eliminated in 1972, (PMID: 6081590)

Sample

source_name

U2OS osteosarcoma cells

cell type

osteosarcoma

passages

unknown

antibody

none -input

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

DNA was extracted with phenol:chloroform and precipitated with 0.1X volume 3M NaOAc, pH 5.2 and 2.5X volume 100% EtOH overnight at -20°C. Preparation of Illumina paired end deep sequencing ChIP libraries was performed as previously described (Yildirim et al., 2011).

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

5410045

Reads aligned (%)

73.7

Duplicates removed (%)

5.0

Number of peaks

334 (qval < 1E-05)

hg19

Number of total reads

5410045

Reads aligned (%)

72.8

Duplicates removed (%)

5.1

Number of peaks

314 (qval < 1E-05)