Progenitor cells from which all blood cells derived. They are found primarily in the bone marrow and also in small numbers in the peripheral blood.
Attributes by Original Data Submitter
hematopoietic stem/progenitor cells from bone marrow
Metadata from Sequence Read Archive
RNA: Total RNA is extracted using the Qiagen Rneasy micro kit. ChIP: ChIP on native chromatin from hematopoitic stem/progenitor cells was performed following previously published protocols (M. Girardot et al., 2014. Nucleic acids research 42, 235-248) with the following modifications using ChIP-grade Magna protein A/G beads (Cat#: 16-663, EMB Millipore). The isolated nuclei were incubated for 15 min at 37°C with 4 units/uL of Micrococcal nuclease (M0247, New England Biolabs) to produce mono/di-nucleosomes as verified by agarose gel electrophoresis. Nucleosomes were precipitated with control IgG, ChIP-grade anti-H4R3me2s (Cat#: Ab5823, Abcam) or anti-H3K9me2s (Cat#: 39375, Active motif). ChIP or input DNA was purified with Ampure XP beads (Cat#: A63880, Beckman Coulter). ChIP libraries were repard according to standard Illumina protocols. Transcriptome libraries were constructed with TruSeq RNA Sample Preparation Kit V2 (Illumia) with minor modifications.