Antigen

Antigen Class

TFs and others

Antigen

E2F1

Cell type

Cell type Class

Prostate

Cell type

LNCAP

Primary Tissue

Prostate

Tissue Diagnosis

Carcinoma

Sample

source_name

LNCaP Cell Line

cell cycle arrest treatment

72 hour CDT

knockdown

shRB

chip antibody

E2F1 (BD-554213 and sc-193)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-Seq was prepared as previously described (Xu et al 2012) ChIP-Seq libraries were constructed ThruPLEX-FD Prep Kit (Rubicon Genomics) with approximately 10 ng of ChIP DNA

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

41853883

Reads aligned (%)

86.8

Duplicates removed (%)

63.6

Number of peaks

25507 (qval < 1E-05)

hg19

Number of total reads

41853883

Reads aligned (%)

86.4

Duplicates removed (%)

64.3

Number of peaks

25234 (qval < 1E-05)