Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
E2F1

Cell type

Cell type Class
Prostate
Cell type
LNCAP
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
LNCaP Cell Line
cell cycle arrest treatment
72 hour CDT
knockdown
shRB
chip antibody
E2F1 (BD-554213 and sc-193)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-Seq was prepared as previously described (Xu et al 2012) ChIP-Seq libraries were constructed ThruPLEX-FD Prep Kit (Rubicon Genomics) with approximately 10 ng of ChIP DNA

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
41853883
Reads aligned (%)
30.9
Duplicates removed (%)
64.3
Number of peaks
25234 (qval < 1E-05)

hg38

Number of total reads
41853883
Reads aligned (%)
31.6
Duplicates removed (%)
63.6
Number of peaks
25507 (qval < 1E-05)

Base call quality data from DBCLS SRA