Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Nup98-96

Cell type

Cell type Class
Larvae
Cell type
3rd instar brain
NA
NA

Attributes by original data submitter

Sample

source_name
brains
developmental stage
3rd instar larva
tissue
brains
chip antibody
Nup98 (Capelson et al., (2010))
genotype/variation
wild type

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates from embryonic cells or 3rd instar brains were clarified from sonicated nuclei and histone-DNA or Nup-DNA complexes were isolated with specific antibody Libraries were prepared according to Illumina's instructions for NextSeq® 500/550 High Output Kit v2 (FC-404-2005).

Sequencing Platform

instrument_model
NextSeq 500

dm3

Number of total reads
43930448
Reads aligned (%)
43.3
Duplicates removed (%)
22.8
Number of peaks
0 (qval < 1E-05)

dm6

Number of total reads
43930448
Reads aligned (%)
41.6
Duplicates removed (%)
25.1
Number of peaks
3278 (qval < 1E-05)

Base call quality data from DBCLS SRA