Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Nup98-96

Cell type

Cell type Class
Cell line
Cell type
S2
Source
Oregon R
Developmental Stage
late embryonic stage

Attributes by original data submitter

Sample

source_name
embryonic cells
developmental stage
embryo
strain
S2
cell type
embryonic cells
chip antibody
Nup98 (Capelson et al., (2010))
genotype/variation
wild type

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates from embryonic cells or 3rd instar brains were clarified from sonicated nuclei and histone-DNA or Nup-DNA complexes were isolated with specific antibody Libraries were prepared according to Illumina's instructions for NextSeq® 500/550 High Output Kit v2 (FC-404-2005).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

dm3

Number of total reads
22142303
Reads aligned (%)
17.5
Duplicates removed (%)
29.9
Number of peaks
3730 (qval < 1E-05)

dm6

Number of total reads
22142303
Reads aligned (%)
16.7
Duplicates removed (%)
32.0
Number of peaks
3073 (qval < 1E-05)

Base call quality data from DBCLS SRA