Antigen

Antigen Class

TFs and others

Antigen

MYC

Cell type

Cell type Class

Neural

Cell type

BE(2)-C

Primary Tissue

Brain

Site of Extraction

Bone Marrow

Tissue Diagnosis

Neuroblastoma

Sample

source_name

Neuroblastoma

cell type

BE2C

chip antibody

n-Myc

antibody catalog

Ab16898

antibody lot

GR291423-1

young_id

20161217_5293

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Purified immunoprecipitated DNA were prepared for sequencing according to a modified version of the Solexa Genomic DNA protocol. Fragmented DNA was end repaired and subjected to 18 cycles of LM-PCR using oligos provided by Illumina. Amplified fragments between 150 and 300bp (representing shear fragments between 50 and 200nt in length and ~100bp of primer sequence) were isolated by agarose gel electrophoresis and purified. Lysates were clarified from sonicated nuclei and target bound fragments were isolated with antibody.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

17714541

Reads aligned (%)

85.8

Duplicates removed (%)

3.9

Number of peaks

21321 (qval < 1E-05)

hg19

Number of total reads

17714541

Reads aligned (%)

85.3

Duplicates removed (%)

4.4

Number of peaks

21327 (qval < 1E-05)