Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K9me2

Cell type

Cell type Class
Embryo
Cell type
4-8h embryos
NA
NA

Attributes by original data submitter

Sample

source_name
embryos
Stage
4-8hr embryo
chip antibody
H3K9me2

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Embryos were collected on standard apple juice plate. Before collection, pre-collection for 1hr was performed. Embryos were then collected for 4 hours and aged for 4 hours in 25C. ChIP was performed following standrad modEncode protocol. Library was prepared using NuGen Ovation Ultralow Library Systems V2

Sequencing Platform

instrument_model
Illumina HiSeq 2000

dm6

Number of total reads
15539375
Reads aligned (%)
92.4
Duplicates removed (%)
5.0
Number of peaks
3853 (qval < 1E-05)

dm3

Number of total reads
15539375
Reads aligned (%)
93.3
Duplicates removed (%)
2.5
Number of peaks
9499 (qval < 1E-05)

Base call quality data from DBCLS SRA