Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
METTL3

Cell type

Cell type Class
Blood
Cell type
MOLM-13
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
Whole cell
cell line
leukemia cell line MOLM13
truseq barcode
ACAGTG
genotype/variation
WT
ip antibody
METTL3
antibody vendor
Bethyl Laboratories
antibody catalog num
A301-568A
antibody lot num
1

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation was performed as previously described (Dawson et al., 2009). Firstly MOLM13 cells were cross-linked with 1% formaldehyde for 10 minutes. 20*10^6 cells were used for each immunoprecipitation with 3ug of specific antibodies or IgG. Immunoprecipitated DNA was purified using the ChIP DNA purification kit (Zymo). Single end 50 bp libraries were prepared using the Bioo Scientific NEXTflex ChIP seq kit following manufacturer's recommendations. Reads were sequenced using HiSeq 1500 and multiplexed reads were split based on their barcodes using Illumina Basespace.

Sequencing Platform

instrument_model
Illumina HiSeq 1500

hg19

Number of total reads
68297583
Reads aligned (%)
46.7
Duplicates removed (%)
51.9
Number of peaks
1314 (qval < 1E-05)

hg38

Number of total reads
68297583
Reads aligned (%)
49.5
Duplicates removed (%)
49.6
Number of peaks
1525 (qval < 1E-05)

Base call quality data from DBCLS SRA