Antigen

Antigen Class

TFs and others

Antigen

HOXB13

Cell type

Cell type Class

Prostate

Cell type

LNCAP

Primary Tissue

Prostate

Tissue Diagnosis

Carcinoma

Sample

source_name

prostate

cell type

Prostate cancer cell line LNCaP

procedure

Chromatin IP against HOXB13

chip antibody

HOXB13 (sc-66923; Santa Cruz)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin immunoprecipitations (ChIP) were performed as described (Stelloo et al. Embo Mol Med 2015) For each ChIP, 5-10μg of antibody prebound to protein A or protein G beads was used. Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Kapa Biosystems kit,KK8234).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg19

Number of total reads

28350781

Reads aligned (%)

98.0

Duplicates removed (%)

3.1

Number of peaks

9704 (qval < 1E-05)

hg38

Number of total reads

28350781

Reads aligned (%)

99.0

Duplicates removed (%)

2.1

Number of peaks

9701 (qval < 1E-05)