Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Blood
Cell type
Monocytes
MeSH Description
Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.

Attributes by original data submitter

Sample

source_name
peripheral blood
cell type
monocytes
developmental stage
old adult
chip antibody
anti-trimethyl-Histone H3 (Lys27) antibody (Millipore catalog no. 07-449)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-seq lysates were clarified from non-fixed Dnase I-treated nuclei and histone-DNA complexes were isolated with anti-trimethyl-Histone H3 (Lys27) antibody (Millipore catalog no. 07-449). For RNA-seq total RNA was extracted from purified monocytes or dendritic cells using RNeasy Micro kit (Qiagen). RNA integrity and concentration were measured using RNA 6000 Nano kits (Agilent) on a Agilent 2100 Bioanalyzer. ChIP-seq libraries were prepared according to Illumina's instructions. RNA-seq libraries were prepared following the Illumina TruSeq RNA low-throughput protocol.

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

hg38

Number of total reads
37893335
Reads aligned (%)
54.5
Duplicates removed (%)
11.9
Number of peaks
2923 (qval < 1E-05)

hg19

Number of total reads
37893335
Reads aligned (%)
54.3
Duplicates removed (%)
12.6
Number of peaks
2850 (qval < 1E-05)

Base call quality data from DBCLS SRA