GSM2476829: T47D-Y537S-E2-TS7-MP2496; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Breast
Cell type
T-47D
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal
Attributes by original data submitter
Sample
source_name
T47D
cell type
T47D
cell subtype - mutation
Y537S
condition
White Medium
treatment
E2
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cell lines before ChIP were then either introduced to White Medium DMEM with or without supplemental E2 or Doxycycline, Cells were sonicated, then cell lysate was incubated with antibody to extract protein-DNA complex of interest, Library Preparation is performed using the Rubicon 48S kit. All of the ChIP-seq performed was done using a combination of Estrogen Receptor ab-10/Thermo Scientific/# MS315PABX and Estrogen Receptor HC-20/Santa Cruz Biotechnolofy/sc-543.