Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MCF7
cell type
MCF7
cell subtype - mutation
Y537S
condition
White Medium
treatment
Doxycycline + E2

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cell lines before ChIP were then either introduced to White Medium DMEM with or without supplemental E2 or Doxycycline, Cells were sonicated, then cell lysate was incubated with antibody to extract protein-DNA complex of interest, Library Preparation is performed using the Rubicon 48S kit. All of the ChIP-seq performed was done using a combination of Estrogen Receptor ab-10/Thermo Scientific/# MS315PABX and Estrogen Receptor HC-20/Santa Cruz Biotechnolofy/sc-543.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
9902440
Reads aligned (%)
98.0
Duplicates removed (%)
10.7
Number of peaks
22598 (qval < 1E-05)

hg19

Number of total reads
9902440
Reads aligned (%)
97.5
Duplicates removed (%)
11.9
Number of peaks
22577 (qval < 1E-05)

Base call quality data from DBCLS SRA