Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Epidermis
Cell type
SK-MEL-147
NA
NA

Attributes by original data submitter

Sample

source_name
Cell culture
cell line
SKmel147
passage
N/A
antibodies
H3K4me3 (Abcam, ab1012)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Native ChIP was done as previously described (Hasson D., et al., The octamer is the major form of CENP-A nucleosomes at human centromeres. Nat Struct Mol Biol. 2013 Jun;20(6):687-95). Libraries for ChIP-seq were done as previously described (Hasson D., et al., The octamer is the major form of CENP-A nucleosomes at human centromeres. Nat Struct Mol Biol. 2013 Jun;20(6):687-95).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
38270199
Reads aligned (%)
97.9
Duplicates removed (%)
20.4
Number of peaks
24529 (qval < 1E-05)

hg19

Number of total reads
38270199
Reads aligned (%)
97.2
Duplicates removed (%)
21.4
Number of peaks
24189 (qval < 1E-05)

Base call quality data from DBCLS SRA